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. 2019 Dec 31;2019:3949824. doi: 10.1155/2019/3949824

Figure 2.

Figure 2

BSZYD promoted the expression of VEGF and VEGFR-2 in HEMECs. (a) HEMECs were incubated in low-serum medium for 24 h and treated with BSZYD for 24 h followed by incubation with or without VEGF (40 ng/mL) for 12 h VEGF and VEGFR-2 levels were determined with western blotting using specific antibodies (left panel); densitometric scanning (right panel). Values are expressed as means ± SD from three independent experiments. P < 0.05 as compared with the control group; #P < 0.05 as compared with the VEGF group. (b) VEGF and VEGFR-2 mRNA level analysis with real-time PCR. Values are expressed as mean ± SD from three independent experiments. P < 0.05 as compared with the control group; #P < 0.05 as compared with VEGF group.