Table 2.
Gene | Primer sequence 5′-3′ | Amplicon |
---|---|---|
VEGF | F: 5′-GGAGGAGGGCAGAATCATCA-3′ | 247 bp |
R: 5′-CTTGGTGAGGTTTGATCCGC-3′ | ||
| ||
VEGFR-2 | F: 5′-TTACTTGCAGGGGACAGAGG-3′ | 170 bp |
R: 5′-TTCCCGGTAGAAGCACTTGT-3′ | ||
| ||
PCNA | F: 5′-GCTCTTGTTCCCTGGATG-3′ | 185 bp |
R: 5′-TTTGGCACCCTCACTTTC-3′ | ||
| ||
CyclinD1 | F: 5′-CCCTCGGTGTCCTACTTCAA-3′ | 219 bp |
R: 5′-CTTAGAGGCCACGAACATGC-3′ | ||
| ||
MMP9 | F: 5′-GAGTTCCCGGAGTGAGTTGA-3′ | 225 bp |
R: 5′-AAAGGTGAGAAGAGAGGGCC-3′ | ||
| ||
GAPDH | F: 5′-CACATCGCTGAGACACCATG-3′ | 198 bp |
R: 5′-TGACGGTGCCATGGAATTTG-3′ |