Fig. 7.

Regulatory network schematic diagram of the sp1418 gene in S. pogona. Blue rectangle: downregulated protein in S. pogona-Sp1418, red rectangle: upregulated protein in S. pogona-Sp1418; green rectangle: transcriptionally downregulated genes in S. pogona-Sp1418, yellow rectangle: transcriptionally upregulated genes in S. pogona-Sp1418. The upregulation of the sp1418 gene affected KatE, RpoC, and InfB, which regulate the redox balance and maintain cell growth, avoiding the effects of oxidative stress on cells. The downregulation of the groEL gene could reduce RNA degradation and maintain RNA stability. SerA converts 3-phosphoglycerate to 3-phosphonooxypyruvate, and PaaZ promotes the synthesis of acetyl-CoA, the upregulation of which could generate more precursors for butenyl-spinosyn biosynthesis, and the upregulation of bus genes promoted biosynthesis. Coproporphyrinogen III oxidase and haem oxygenase are related to secondary metabolism, the downregulation of which could provide additional raw materials for butenyl-spinosyn biosynthesis. The expression change in genes involved in sporulation revealed differences in phenotypes and is also associated with primary and secondary metabolism