Fig. 4.

USP22 promoted tumor growth in nude mice. a BALB/c nude mice were randomly divided into 4 groups (n = 8/group). USP22-overexpressing SGC7901 cells, USP22-silenced AGS cells, or corresponding negative control cells were subcutaneously inoculated into the right armpit of each mouse. b Mice were sacrificed at 30 d after inoculation. Tumors were immediately harvested. c Tumor volume (V) was measured twice a week after tumor formation and calculated as ½ × length × width². d, e Tumor tissue sections were subjected to immunohistochemical staining for USP22, Ki-67, and TUNEL expression. Images were acquired at magnification ×100. Representative images are shown