Fig. 5.

USP22 overexpression increased son of sevenless 1 (SOS1) expression and activation of the RAS/ERK and PI3K/AKT pathways. a USP22-overexpressing SGC7901 cells and negative control cells were subjected to a chromatin immunoprecipitation assay. qRT-PCR was performed to validate the differential expression of some genes. *P < 0.05 vs. NC; n = 3. b Western blot analysis was performed to determine the protein levels of USP22, SOS1, Ras, ERK, p-ERK, c-myc, PI3K, AKT, p-AKT, BAD, and bcl-2 in USP22-overexpressing SGC7901 cells and tumor tissue. GAPDH was used as an internal control