Fig. 7.

Exo-miR-15b-3p regulates tumor growth in vivo. a miR-15b-3p expression levels in BGC-823 cells (stably transfected with Lv-miR-15b-3p/Lv-NC or Lv-inhibitor/Lv-inNC) or exosomes isolated from BGC-823 cells were detected using qRT-PCR. SGC-7901 cells were treated with PBS or exosomes loaded with Lv-miR-15b-3p/Lv-NC or Lv-inhibitor/Lv-inNC and were subsequently injected into the nude mice (n = 5). The xenografts (b) and tumor growth curve (c) show that Exo-Lv-miR-15b-3p promotes, while Exo-Lv-inhibitor suppresses xenograft tumor growth in nude mice. d Representative images of tumor growth of the mice treated with exosomes derived from stably transfected-BGC-823 cells or PBS, were determined using luciferase-based bioluminescence imaging. e Representative images of TUNEL staining of the xenograft tumors for the ectopic expression or silencing of Exo-miR-15b-3p and their corresponding control or PBS groups. Scale bar, 100 μm. f Quantification of TUNEL-positive cells. g qRT-PCR analysis of miR-15b-3p and DYNLT1 expression in xenograft tumors with the treatment indicated. h Immunohistochemical analysis of DYNLT1 expression in the xenografts. Scale bar, 50 μm. i Western blotting analysis of DYNLT1, BAX, BCL-2, Cleaved Caspase-9 and Cleaved Caspase-3 in xenograft tumor tissues among the different groups. The internal control used was β-Actin. Mean ± SEM of the results are presented