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. 2020 Feb 10;13:22. doi: 10.1186/s12920-020-0692-3

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — STAiR18 knockdown affects the vitality of INA-6 cells. Permanently IL-6-treated INA-6 cells were transfected with siRNAs targeting STAiR18 exons 1, 2 and 3, as well as a negative control siRNA. To compare the knockdown effects on cell vitality, a STAT3 knockdown was also conducted. Cell vitality was analyzed by determining Annexin-V- and PI-stained cell populations via flow cytometry (first panel), cellular ATP content using the CellTiter-Glo-kit (middle panel) and Caspase3-assay (third panel) after 24 h, 48 h and 72 h (n = 3). The indication of significances refers to the control