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. 2020 Feb 8;9:6. doi: 10.1186/s40035-020-0184-6

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — The comparison of FiNPCs and AiNPCs

a Photographs of identical fields of neurospheres were taken for FiNPCs and AiNPCs after cultured in proliferation conditions for 3 days. b The number and size of neurospheres in each field were measured. c Cells expressing Sox2, Nestin or Ki67 specific immunoreactivities in the FiNPCs and AiNPCs groups. d The proportions of Sox2⁺, Nestin⁺, or Ki67⁺ cells in each field were counted. e The transcript expression of Sox2, Nestin and Ki67 was determined by qPCR analysis. f Cells expressing Tuj1 or GFAP specific immunoreactivities in the FiNPCs and AiNPCs groups after cultured in differentiation conditions for 3 days. g The proportions of cells expressing Tuj1 or GFAP in each field were counted. h The transcript expression of Tuj1 and GFAP was determined by qPCR analysis. i Cells expressing vGlut, GABA or ChAT specific immunoreactivities in the FiNPCs and AiNPCs groups after cultured in differentiation conditions for 7 days. j The proportions of cells expressing vGlut, GABA or ChAT in each field were counted. k Photographs of identical fields of cells were taken for the FiNPCs and AiNPCs groups at 0 h and 24 h in wound healing assay. l The total number of invading cells of each field was counted and represented in fold change. m Photographs of identical fields of DAPI labeled cells were taken for the FiNPCs and AiNPCs groups after cultured in transwells for 12 h. n The total number of migrated cells of each field was counted and represented in fold change. o, q Photographs of identical fields of TUNEL⁺ cells were taken for the FiNPCs and AiNPCs groups after cultured in proliferation o and differentiation q conditions. p, r The total number of TUNEL⁺ cells in proliferation p and differentiation r conditions was counted and represented in proportions. Scale bars represent 50 μm (a, c, f, i, o, q), 400 μm (k), and 100 μm (m). Data were normalized to GAPDH and presented as fold change. Error bars denote s.d. from triplicate measurements. *P < 0.05, **P < 0.01, ***P < 0.001, and ***P < 0.0001 by two-tailed t test (n = 3).