Fig. 5.

Evans blue extravasation and fibrinogen. Panel A, Evans blue staining. i-iv show the brain of 4 infected rats, arrows show cysts, arrowhead shows blue staining surrounding the cyst. v, magnification of iii, shows red fluorescence in the border of the cyst (scale bar, 1mm). vi, magnification image shows Evans blue extravasation in the parenchymal tissue (evans blue in red, DAPI in blue, scale bar 50μm). Panel B, Fibrinogen presented in fibrosis. i-iii represent non-infected brain, no evidence of fibrogen or fibrosis is observed. iv-vi shows infected brain, fibrinogen is observed in the fibrotic tissue, confirmed by Masson’s trichrome staining. Images at low magnification i and iv, scale bar 200μm. High magnification images, scale bar 50μm. vii, shows fibrinogen inmunoreactive area by different cyst location. Control region (Cp, [n=6]), parenchymal cyst (P, [n=11]), corticomeningeal cyst (Cm, [n=7]), control region of the ventricle (Cv, [n=5]), ventricular cyst (V, [n=7]). Mann-Whitney U, P<0.05 (*), P<0.001 (**), P<0.0001 (***)