Fig. 8.
Effect of IRF3 or IRF7 deficiency on SINV-induced cell death in brain and spinal cord. WT, IRF3−/− and IRF7−/− mice were infected intracranially with 103p.f.u. SINV TE. Representative TUNEL-stained sections of brain (a) and spinal cord (b) from uninfected (d0) and SINV-infected WT, IRF3−/− and IRF7−/− mice at 5 and 7 days p.i. Two hundred× magnification; the scale bar represents 100 µm. TUNEL-positive cells have dark brown nuclear staining. The numbers of TUNEL-positive cells were quantified on coded slides from brains (c) and spinal cords (d). The data represent the results of two independent experiments, totalling four mice per strain per time point. **P<0.01, ***P<0.001, Tukey’s multiple comparison test.