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. 2019 Sep 17;100(11):1515–1522. doi: 10.1099/jgv.0.001319

Fig. 4.

Fig. 4.

DENV-induced morbidity and mortality in a murine model of dengue disease are correlated with increasing levels of Sia and NS1 in circulation. (a) Ifnar —/— mice were infected with DENV2-D220, and 3 days post-infection, circulating levels of Sia were measured by (b) ELISA and (c) a newly developed LC-MRM-MS assay. The latter approach showed that serum levels of free N-glycolylneuraminic acid (Neu5Gc), a form of Sia found in most non-human mammals, were significantly higher in DENV-infected mice. (d) High levels of NS1 and Sia were associated with increased mortality in DENV-infected mice. These findings indicate that DENV infection leads to disruption of EGL components, such as Sia, which can be measured in serum and could used as potential biomarkers for severe dengue disease in humans.