Table 1.
Optimized conditions for sialic acids in multiple reaction monitoring detection
|
Compound name |
Precursor ion |
Product ion |
Fragmentor (V) |
Collision energy (V) |
Polarity |
|---|---|---|---|---|---|
|
N-acetylneuraminic acid |
308.1 |
170 |
80 |
5 |
Negative |
|
|
308.1 |
98 |
80 |
20 |
Negative |
|
|
308.1 |
87* |
80 |
5 |
Negative |
|
N-glycolylneuraminic acid |
324.1 |
186 |
80 |
10 |
Negative |
|
|
324.1 |
116 |
80 |
10 |
Negative |
|
|
324.1 |
87* |
80 |
15 |
Negative |
|
[13C3]-N-acetylneuraminic acid |
311.1 |
173 |
80 |
5 |
Negative |
|
|
311.1 |
99 |
80 |
20 |
Negative |
|
|
311.1 |
90* |
80 |
5 |
Negative |
*Quantifier ion