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. Author manuscript; available in PMC: 2020 Feb 11.
Published in final edited form as: ACS Chem Neurosci. 2018 Jun 1;9(11):2701–2712. doi: 10.1021/acschemneuro.8b00144

Figure 1.

Figure 1.

PP1α and PP1γ1 are both endogenously expressed in HEK293 cells, and overexpression of PP1 regulates endogenous PP1 expression. (A) Myc-tagged PP1α or PP1γ1 was overexpressed in HEK293 cells and immunoblotted with a Myc antibody, a PP1α selective antibody, or a PP1γ1 selective antibody. (B) The PP1α selective antibody was ~31.3-fold more robust at detecting PP1α over PP1γ1 when normalized to Myc signal. Conversely, the PP1γ1 selective antibody was 13.5-fold more robust at detecting PP1γ1 over PP1α. (C) Upon normalizing to Myc intensity (to normalize for differences in antibody detections) and loading, the PP1α and PP1γ1 antibodies detected equal concentrations of PP1α and PP1γ1. (D) PP1α overexpression increased the expression of endogenous PP1α but decreased the expression of endogenous PP1γ1. Conversely, PP1γ1 overexpression increased the expression of endogenous PP1γ1 but decreased the expression of endogenous PP1α. A 1-sample t-test comparing to a theoretical value of 1 was performed. *p < 0.05, ***p < 0.001.