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. Author manuscript; available in PMC: 2020 Feb 11.
Published in final edited form as: ACS Chem Neurosci. 2018 Jun 1;9(11):2701–2712. doi: 10.1021/acschemneuro.8b00144

Figure 4.

Figure 4.

Spinophilin association with PP1 was not regulated by Ser17 mutations of spinophilin. HEK293 cells were transfected with WT, S17A, or S17D spinophilin and immunoprecipitated for spinophilin. Lysates and immunoprecipitates were immunoblotted for spinophilin and either (A) PP1α or (B) PP1γ1. There was no effect of the mutations on spinophilin association with PP1. (C) HEK293 cells were transfected with WT or S17A spinophilin in the absence or presence of CDK5/p35 and immunoprecipitated for spinophilin. Lysates and immunoprecipitates were immunoblotted for spinophilin and PP1α. S17A mutation had no effect on the CDK5-dependent increase in spinophilin binding to PP1. For lysates, 0.5% (A and C) or 1.33% (B) of the total was loaded for spinophilin and PP1 blots. For immunoprecipitates, 25% (A and C) or 33% (B) of the immunoprecipitate was loaded. A one-way (A and B) or a two-way ANOVA (C) was performed.