Table 2.

Mean frequency of micronucleated binucleated cells (MNBC) in CHO-K1 and HeLa cells obtained in the MN test after 3 and 24 h of treatment with indirubin.

	CHO-K1				HeLa
	MNBC (3 h)		MNBC (24 h)		MNBC T (24 h)
Treatments	Mean ± SD	IF	Mean ± SD	IF	Mean ± SD	IF
PBS	7.33 ± 1.53	–	8.00 ± 1.00	–	17.33 ± 6.43	–
DXR 0.75 μg/mL	171.00 ± 10.15*	23.32	207.00 ± 13.90*	25.88	26.00 ± 1.73	1.50
IRN 0.1 μM	9.67 ± 3.22	1.32	12.00 ± 2.65	1.50	14.00 ± 5.29	0.81
IRN 0.5 μM	11.00 ± 2.65	1.50	6.33 ± 0.58	0.79	28.33 ± 1.53	1.63
IRN 1.0 μM	9.33 ± 0.58	1.27	7.00 ± 1.00	0.88	21.00 ± 3.61	1.21
IRN 5.0 μM	9.33 ± 2.31	1.27	10.00 ± 1.00	1.25	25.33 ± 2.52	1.46
IRN 10.0 μM	11.00 ± 2.00	1.50	9.00 ± 1.00	1.13	23.67 ± 11.06	1.37

SD: standard deviation; PBS: phosphate-buffered saline, negative control; DXR: doxorubicin, positive control; IRN: indirubin; IF: induction factor.

^*Indicates significant difference compared to the negative control group (p < 0.05, ANOVA followed by Tukey’s test).