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. 2019 Sep 12;4(1):pkz067. doi: 10.1093/jncics/pkz067

Figure 1.

Figure 1.

Outline of the panel of DNA Repair assays used to determine the personal DNA Repair score. Each assay measures the activity of a specific DNA Repair enzyme, in a protein extract prepared from PBMC, to remove its target DNA damage from a synthetic short oligonucleotide, shown schematically in the upper panel. Each enzyme assay was run separately under optimized conditions. The target damaged base in each substrate oligonucleotide was 8-oxoguanine in the OGG1 assay (illustrated by a black circle), hypoxanthine in the MPG assay (orange square), and a furanyl abasic site in the APE1 assay (blue triangle). The asterisks represent a 3'-Yakima Yellow fluorescence tag. Assays were run on a robotic platform and analyzed in an AB3130XL genetic analyzer. The personal OGG1, MPG, and APE1 enzyme activities were then used to calculate the personal DNA Repair score for each participant, as described in Methods. Further description of the DNA Repair assays is provided in the Supplementary Materials (available online). APE1 = apurinic/apyrimidinic endonuclease 1; MPG = methylpurine DNA glycosylase; OGG1 = 8-oxoguanine DNA glycosylase; OMA = OGG1, MPG, and APE1; PBMC = peripheral blood mononuclear cells.