Fig. 4. (A) Cartoon representation of MCF-7 cells co-cultured/incubated with autofluorescent PNVCL at different temperatures. (B) CLSM images of MCF-7 cells labelled with PNVCL (250 μg mL^–1) at 25, 35, and 38 °C for 24 h. Scale bars = 10 μm. (C) Temperature governed fluorescence intensities of PNVCL treated MCF-7 cells were measured and expressed as a ratio of intensity of blue, green and red fluorescence in respect of blue fluorescence emitted by 4′,6-diamidino-2-phenylindole (DAPI) only. Data are expressed as the mean of triplicate determinations (mean ± standard error of mean (SEM)), represented by vertical bars (*P < 0.05).