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. 2019 Oct 30;21(2):178–188. doi: 10.1080/15384047.2019.1679555

Figure 1.

Figure 1.

Triptolide affects the viability of leukocyte subsets and tumor cells in vitro. (a) The cell viability of macrophages, neutrophils, lymphocytes and 4T1 mammary tumor cells after treatment with triptolide for 48 h was assessed by CCK8 analysis. The results are presented as the means from three different experiments. (b) The in vitro-differentiation of macrophages was stimulated with LPS (100 ng/mL) + IFN-γ (500 IU/mL) (M1) and IL-4 (20 ng/mL) (M2) in the presence or absence of triptolide for 48 h. (c) Macrophages derived from blood monocytes and treated with different concentrations of triptolide were stained with Annexin V and PI and analyzed by flow cytometry. (d) Effect of triptolide on the level of the apoptosis-related protein poly (ADP-ribose) polymerase (PARP) in macrophages derived from blood monocytes and treated with different concentrations of triptolide. The levels of PARP were assessed by Western blotting. *P < .05, **P < .01 and ***P < .001 compared with the macrophage (a) or CTRL group (b) (as determined by student’s t-test).