Table 1. Composition of the Complete MUC1-Tn PS-SGCL Librarya.
| fixed position P(Y) | no. of sublibraries | sublibrary sequence | number of glycopeptides/sublibrary |
|---|---|---|---|
| P4 (T* or T) | 2 | HGVT*XAPDXRPAPGXXAPPA (11) | 16 |
| HGVTXAPDXRPAPGXXAPPA (12) | 16 | ||
| P5 (S* or S) | 2 | HGVXS*APDXRPAPGXXAPPA (13) | 16 |
| HGVXSAPDXRPAPGXXAPPA (14) | 16 | ||
| P9 (T* or T) | 2 | HGVXXAPDT*RPAPGXXAPPA (15) | 16 |
| HGVXXAPDTRPAPGXXAPPA (16) | 16 | ||
| P15 (S* or S) | 2 | HGVXXAPDXRPAPGS*XAPPA (17) | 16 |
| HGVXXAPDXRPAPGSXAPPA (18) | 16 | ||
| P16 (T* or T) | 2 | HGVXXAPDXRPAPGXT*APPA (19) | 16 |
| HGVXXAPDXRPAPGXTAPPA (20) | 16 |
a
Y = fixed position of glycosylated (T*/S*) or nonglycosylated (T/S) building block, X = randomized position, and T*/S* corresponds to Thr/Ser bearing Tn antigen). Each sublibrary holds one position constant for either T*/S* or T/S, while the remaining positions are randomized (X).