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. 2020 Feb 6;3(3):e201900526. doi: 10.26508/lsa.201900526

Figure 2. MHV68-infected germinal center cells express lambda light chain more frequently.

Figure 2.

Graph shows the percentage of cells expressing Kappa (Vκ), Lambda (Vλ), or both light chains from cells where the matching heavy chain was also identified. Single MHV+ and MHV− germinal center cells were isolated from the spleens of intranasal MHV68-inoculated mice 17 days postinfection on the basis of YFP+ or YFP− expression. Analysis of expression was by RT-PCR and sequencing of individual cells. Five independent biological replicates were analyzed with replicate sample 1 consisting of two pooled mice and samples 2 through 5 from individual mice. The mean and SEM are displayed. t test, ***P < 0.001.