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. 2020 Feb 6;3(3):e201900526. doi: 10.26508/lsa.201900526

Figure 7. Reactivity of immunoglobulins to self and viral antigens.

Figure 7.

(A) ELISA test for reactivity against nuclear antigens. Graphs show OD405 values at 0.01, 0.1, and 0.01 μg/ml antibody concentrations of recombinant antibodies from MHV+ and MHV− populations. Anti-DNA antibody-positive control BV17-45 (dashed line) and negative control anti-myc antibody are displayed. The mean and SEM are displayed for each antibody tested in duplicates. (B) Antiviral specificity of antibodies. Flow cytometry analysis of cells infected with MHV68 and stained with recombinant antibodies. Plot shows percent of MHV infected (YFP+) cells reactive with individual recombinant antibodies from indicated populations. Positive control antibody that recognizes ORF46 (MHV68UNG) and negative control anti-myc are indicated. Mann–Whitney test, **P < 0.01.