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MiR-421 promotes the growth of osteosarcoma cells (a) After transfecting with miR-421 mimic/inhibitor, the proliferations of MG63 and U2OS cells were detected by MTT assay. Data are shown as mean ± SEM. ** P < .05(b-e) After transfecting miR-421 mimic/inhibitor in MG63 and U2OS cells, the levels of Cyclin D1 and bcl-2 were detected by western blot and real-time PCR. Data are shown as mean ± SEM. ** P < .05.
