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MiR-421 promotes the release of IL-6 in osteosarcoma cells (a, b) After transfecting miR-421 mimic/inhibitor in MG63 and U2OS cells, the expression of IL-6 was detected by ELISA. (c-f) After transfecting miR-421 mimic/inhibitor in MG63 and U2OS cells, the levels of IL-6 were detected by western blot and real-time PCR. Data are shown as mean ± SEM. ** P < .05.
