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. 2019 Oct 8;21(1):17–25. doi: 10.1080/15384047.2019.1647050

Figure 1.

Figure 1.

Up-regulated DLX6-AS1 promotes cell proliferation, migration and EMT in gastric cancer. (a) qRT-PCR analysis of the expression level of DLX6-AS1 in gastric cancer cell lines (AGS, HGC-27, BGC-823 and SGC-7901) and normal gastric epithelial cell GES-1. (b) DLX6-AS1 levels in AGS and HGC-27 cells with sh-DLX6-AS1#1, sh-DLX6-AS1#2 or sh-DLX6-AS1#3 treatment, compared with sh-NC group, were tested through qRT-PCR experiment. (c) CCK-8 assay was performed to detect the proliferative ability of DLX6-AS1-silenced AGS and HGC-27 cells. (d) Cell migration capacity of AGS and HGC-27 cells treated with sh-DLX6-AS1#1 was measured by transwell migration assay. (e) The expression levels of EMT-associated proteins, including E-cadherin, N-cadherin and Vimentin, in DLX6-AS1#1-transfected AGS and HGC-27 cells as respectively estimated by western blot. *P < .05, **P < .01.