MAP4K1 highly expressed in GC is regulated by DLX6-AS1 in a positive way. (a) Western blot results of the expression of five MAP4K family members: MAP4K1, MAP4K2, MAP4K3, MAP4K4 and MAP4K5, under the transfection of sh-DLX6-AS1#1. (b) The expression level of MAP4K1 in GC cell lines and normal gastric epithelial cell was individually assessed by qRT-PCR. (c) qRT-PCR analysis of the levels of MAP4K1 in AGS and HGC-27 cells with the silencing of DLX6-AS1. *P < .05, **P < .01, ***P < .001.