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. 2019 Oct 8;21(1):17–25. doi: 10.1080/15384047.2019.1647050

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Figure 4. — DLX6-AS1 stabilizes MAP4K1 by regulating FUS. (a) The binding of FUS and MAP4K1 from starbase v2.0. (b) RIP assay was conducted to confirm the combination of FUS with MAP4K1. (c) qRT-PCR analysis of the high FUS expression in GC cell lines. (d-e) The mRNA and protein levels of FUS in AGS and HGC-27 cells under sh-DLX6-AS1#1 treatment were evaluated by qRT-PCR. (f) AGS and HGC-27 cells were co-transfected with sh-DLX6-AS1#1 and pcDNA3.1 or sh-DLX6-AS1#1 and pcDNA3.1-FUS, with sh-NC and pcDNA3.1 as negative control. The efficiency of transfection was examined by qRT-PCR experiment. (g) Under actinomycin D treatment, the mRNA level of MAP4K1 with different treatments was dissected by qRT-PCR. *P < .05, **P < .01.