TRIM58 inhibition of GC cell proliferation may be due to β-catenin ubiquitination and inactivation After treatment with shTRIM58 lentivirus and XAV939 in SNU719 cells, (A) Cell proliferation was assessed at 0, 24, 48, and 72 h, and (B) the levels of related proteins (β-catenin, C-myc, Cyclin D1 and survivin) were analyzed by Western blot. (C) A Co-IP shows the co-existence of TRIM58 and β-catenin in the precipitant. (D) β-catenin ubiquitination was examined after infection with TRIM58 lentivirus, and β-catenin expression was detected by western blot. **P < .01 and ***P < .001 compared to shNC + DMSO, ##P < .01 and P < .001 compared to shTRIM58 + DMSO, and ++P < .01 and +++P < .001 compared to shNC + XAV939.