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. 2019 Dec 18;15(1):1703503. doi: 10.1080/15592324.2019.1703503

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Figure 1. — Characterization of the oligosaccharide fraction that stimulates the auxin-induced development of adventitious roots on the buckwheat hypocotyl. (a) Chromatography of the carbohydrate fraction obtained from the buffer-extractable compounds of pea seedlings on Biogel P-4, revealing a fraction of oligosaccharides that stimulates auxin-induced rhizogenesis (OSRG). (b) The increase in the number of auxin-induced adventitious roots per hypocotyl in the presence of OSRG (means ± standard error). (c) Absorption spectra obtained for buffer-extractable compounds before purification for the mixture of standard monosaccharides with a composition similar to that of OSRG and for the collected oligosaccharide fraction. (d) Determination of the molecular weight distribution (according to pullulan standards) using sequentially connected OHpak SB-806M HQ and OHpak SB-804 HQ columns. (e) Monosaccharide analysis of the collected oligosaccharide fraction performed by high-performance anion-exchange chromatography.