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Quantification of extracellular HBV DNA in Hep38.7-Tet cell cultures using IC-RT-qPCR assay. (A) The layout of IC-RT-qPCR assay. (B) The immunoplate was coated with anti-HBs antibody or normal rabbit IgG. Culture supernatants from Hep38.7-Tet cells were added into the antibody-coated wells. HBV DNA amounts in the wells were quantified using qPCR. Data represent mean ± SD of triplicate wells.
