Representative immunostaining of (a) IL-22-expressing cells, (b) IL-17A-expressing CD4 T cells, (c) IL-4-expressing CD4 T cells, (d) IFN-γ-expressing CD4 T cells, (e) Foxp3-expressing CD25 T cells, (f) IL-10-expressing CD20 B cells, and (g) IDO-expressing CD123 plasmacytoid dendritic cells in tissue biopsies from subacute cutaneous lupus erythematosus ((A) SCLE), discoid lupus erythematosus ((B) DLE), healthy donors ((C) HD). Arrowheads show single staining (in brown or red), and arrows depict double staining (in burgundy). Original magnification was ×600. (h–n) Percentage of immunoreactive cells per microscopic field. The results are expressed as the mean (horizontal yellow line), median (horizontal black line), and 5th/95th percentiles. ∗P < 0.05 and ∗∗P < 0.001. SCLE: subacute cutaneous lupus erythematosus; DLE: discoid lupus erythematosus; HD: healthy donors.