Figure 2.
Effect of ASC concentration on viability and proliferation in DAT hydrogel. ASCs were seeded in the hydrogels at concentrations of 0.25 × 106, 0.5 × 106, 1 × 106, and 2 × 106 cells/mL and cultured in stromal media. Analysis of viability was performed on day 1 (1 day after seeding), day 4, day 7, day 14, and day 21. (a) Representative images of calcein AM stained viable cells (green); (b) ASC proliferation determined from fluorescence intensity (reduced alamar blue) for each time point relative to day 1 (fold). Data are expressed as mean (n = 4) ± SD; level of significance: (∗) p < 0.05; Scale bar 1000 μm.