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. 2019 Dec 27;2019:9276398. doi: 10.1155/2019/9276398

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Copyright © 2019 Omair A. Mohiuddin et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Analysis of MMP-2 expression by gelatin zymography and qRT-PCR. (a, b) Representative images of Pro MMP-2 and MMP-2 bands observed after gel electrophoresis of 14 (a) and 28 (b) day samples. (c) Pro MMP-2 and MMP-2 band area was determined by densitomteric analysis using ImageJ software, data are presented as fold change relative to the control samples. (d) Ratio of MMP-2 band area to Pro MMP-2 band area determined by densitometric analysis. (e) qRT-PCR analysis of MMP2 mRNA expression in control, adipogenic, and osteogenic samples are displayed as fold change relative to the control samples. Data are expressed as mean (n = 6) ± SD; level of significance: (^∗) p < 0.05.