FIGURE 5.

Tofacitinib reduces the number of apical intercellular gaps via ZO-1 rearrangement/localization caused by IFN-γ. A, Pretreatment: T₈₄ cells grown on coverslips pretreated with tofacitinib or DMSO for 1 hour followed by IFN-γ (1000 U/mL, 24 h) treatment were stained for ZO-1 and visualized via immunofluorescence microscopy. B, Average number of intercellular gaps in 5 fields of view per condition from 3 independent pretreatment experiments (**** P < 0.0001, ### P < 0.001, ## P < 0.01, n = 3). C, Post-treatment: ZO-1 staining in cells treated with tofacitinib or DMSO at 6 hours and 18 hours post-IFN-γ (1000 U/mL, 24 h) treatment. D, Number of intercellular gaps taken in 10 fields of view per condition from 3 independent post-treatment experiments (**** P < 0.0001, ### P < 0.001, n = 3). White arrows indicate intercellular gaps.