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. Author manuscript; available in PMC: 2020 Feb 11.
Published in final edited form as: Hear Res. 2018 Jul 10;367:59–73. doi: 10.1016/j.heares.2018.07.006

Fig. 7.

Fig. 7.

Ouabain blocks the interaction between α1 and β1 subunits. Cells were treated with 5 μM ouabain and activity was measured up to 72 hrs. Cell lysates were immunoprecipitated (IP) with anti-β1 antibody (a) or anti-α1 antibody (b) and immunoblotted (IB) with anti-α1 or anti-β1 antibody. The signals of α1 and β1 subunits disappeared due to the ouabain treatment compared to non-treatment. IgG used for co-immunoprecipitation with cell lysate extraction is a negative control. Ext: cell lysate extraction. Input: cell lysate without IP.