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. 2020 Feb 7;13:1159–1170. doi: 10.2147/OTT.S232354

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© 2020 Ma et al.

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LncRNA PCAT6 could sponge miR-543. (A) The binding sites between miR-543 and lncRNA PCAT6 were predicted by Starbase online software. (B and C) Luciferase activity was determined in SiHa and HeLa cells co-transfected with PCAT6-WT or PCAT6-MUT and miR-NC or miR-543. (D) RNA-pull down experiment was conducted to verify the combination between miR-543 and lncRNA PCAT6 in CC cells. (E and F) The combination between miR-543 and lncRNA PCAT6 was confirmed by RIP experiment. (G and H) The overexpression or knockdown efficiency of PCAT6 overexpression plasmid or small interfering RNA against PCAT6 (si-PCAT6#1, si-PCAT6#2, si-PCAT6#3) was assessed in SiHa and HeLa cells by qRT-PCR. (I) The expression of miR-543 was examined in SiHa and HeLa cells transfected with Vector or PCAT6 by qRT-PCR. (J) QRT-PCR was performed to detect the abundance of miR-543 in the two CC cells transfected with si-NC or si-PCAT6#1. *P<0.05.