Table 2. Expression levels of four chosen genes in leaves of healthy and inoculated plants of wheat variety Bohemia and healthy V2-49-17 and Scorpion varieties.
The expression of four genes (B2H2, ICS, PAL, and PR2) were detected in healthy and inoculated Bohemia, V2-49-17 variety with yellow endosperm, and cv. Scorpion with blue aleurone layer in different treatment conditions (ergosterol, aescin, sodium bicarbonate, prochloraz and potassium iodide) and indicated as a fold difference (FD) to control (treatment water).
| Variant | Gene | Ergosterol | Aescin | Sodium bicarbonate | Prochloraz | Potassium iodide | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Healthy Bohemia | B2H2 | -0,99 | *** | 10,16 | **** | 838,05 | **** | 72,96 | **** | 7370,13 | **** |
| ICS | -0,76 | *** | 3,76 | **** | 2,71 | *** | 4,53 | **** | -0,39 | *** | |
| PAL | -0,83 | ** | 0,94 | ** | 5,99 | **** | 5,06 | **** | 4,98 | **** | |
| PR2 | -0,84 | ** | 3,97 | **** | 1,07 | ** | 0,42 | ** | 20,75 | **** | |
| Inoculated Bohemia | B2H2 | 2,29 | ** | 2,80 | ** | 0,95 | ** | 5,20 | **** | 7,45 | **** |
| ICS | -0,27 | 1,33 | *** | 0,41 | ** | -0,85 | *** | -0,57 | ** | ||
| PAL | 2,10 | ** | 1,98 | ** | 4,99 | **** | 7,12 | **** | 3,86 | **** | |
| PR2 | 1,30 | *** | 3,11 | **** | 1,96 | *** | 1,31 | *** | 30,80 | **** | |
| V2-49-17 variety with yellow endosperm | B2H2 | 42,73 | **** | 46,67 | **** | 1060,53 | **** | 2040,09 | **** | 1362,64 | **** |
| ICS | 1,21 | **** | 8,27 | **** | 6,26 | **** | 12,29 | **** | -0,33 | *** | |
| PAL | 8,57 | *** | 4,39 | *** | 57,79 | **** | 82,05 | **** | -1,00 | * | |
| PR2 | 23,42 | **** | 58,49 | **** | 25,16 | **** | 20,09 | **** | 48,52 | **** | |
| Scorpion with blue aleurone layer | B2H2 | -0,98 | **** | -0,98 | **** | 122,92 | **** | -0,42 | *** | 44,48 | **** |
| ICS | 1,22 | **** | -0,69 | *** | -0,87 | *** | 1,59 | **** | -0,09 | * | |
| PAL | 5,85 | *** | -0,02 | 14,02 | **** | 43,83 | **** | -1,00 | *** | ||
| PR2 | 4,08 | **** | 2,73 | **** | 20,16 | **** | 21,71 | **** | 49,85 | **** | |
Significant fold differences between water control and experimental treatments are indicated by asterisks (P < 0.05 (*); P < 0.01 (**); P < 0.001 (***); P < 0.0001 (****). The statistical analyses were carried out separately within each gene and treatment.