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. 2019 Jun 13;105(2):508–518. doi: 10.3324/haematol.2018.212118

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Figure 2. — Readthrough agents (RTA) can increase FVIII antigen levels in the supernatants of some transfected Chinese hamster ovary (CHO) cells. Human FVIII:Ag levels analyzed by ELISA in the supernatants of CHO cells transiently transfected with the F8 variants, showing the increase in FVIII:Ag levels before and after RTA-treatment versus the F8 wild-type (WT) variant. BDD-FVIII: WT; CT: untreated controls; GN100: 100 mg geneticin/mL and GT100: 100 mg gentamicin/mL. ^*P<0.05; ^**P<0.01; ^***P<0.001. (N=3).