Figure 6.

3F_ax-Neu5Ac treatment impairs adhesion and rolling of MM1S^Heca452 on E-selectin, VCAM1 and MADCAM1 under shear stress. MM1S^Heca452 cells were treated with 300 mM 3F_ax-Neu5Ac or dimethyl sulfox-ide (DMSO) (vehicle control) for seven days. After treatment, cells were collected, washed and resuspended at 2x10⁶/mL. Eighty μL of cell suspension were loaded onto E-selectin- (A-C), MADCAM1- (D-F) and VCAM1-(G) coated microfluidic channels and adhesion/rolling assay was performed at 0.5 dyne/cm² at RT using the Mirus Evo NanoPump. Rolling cells were imaged using an A-Plan 10X/0.25 objective of an A10 Vert.A1 microscope equipped with a QIClick F-M-12 Mono camera. Images were acquired using the Vena Flux Assay software and analyzed using the Image-Pro Premiere. Bars represent the mean±Standard Error of Mean of three independent experiments. Unpaired Student’s t-test was used to determine statistical significance. *P<0.05; **P<0.01; ns: non-significant.