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. 2019 May 23;105(2):435–447. doi: 10.3324/haematol.2019.216986

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Figure 2. — High STAT5A activation promotes CD8⁺ T-cell neoplasia. (A) Flow cytometric analysis of splenocytes of diseased cS5A^hi mice and wildtype (wt) littermates showing absolute CD8⁺ T-cell (P=0.0001), CD4⁺ T-cell (P=0.0002), B-cell (P≤0.0001, all unpaired t-test with the Welch correction), CD11b⁺Gr1^hi (P=0.0014) and natural killer (NK)-cell numbers (P=0.0237, both unpaired t-test) (B) Left: tumor weight of subcutaneous (s.c.) MC-38 tumors 18 days after injection of 1x10⁶ cells in both flanks of 10-week old wt (n=11), cS5A^lo (n=9) and cS5A^hi (n=10) mice (one-way analysis of variance with the Tukey multiple comparison test). Middle: macroscopic view of isolated MC-38 tumors, scale bar represents 1 cm. Right: tumor incidence per injection of MC-38 cells (logistic regression, P=0.031) and percentage of CD8⁺ T-cell tumor infiltrating cells (Kruskal-Wallis test with the Dunn multiple comparison test). (C) Flow cytometric analysis of CD2, CD3 and CD5 expression on CD8⁺ wt (n=10) and cS5A^hi (n=11) splenocytes. Mean fluorescent intensity (MFI) unpaired t-test (CD2 P=0.85, CD3 P=0.91, CD5 P=0.0002), relative expression unpaired t-test with the Welch correction (CD2 P=0.0001, CD3 P=0.0044, CD5 P=0.0021). (D) Flow cytometric characterization of splenic wt (n=13) and cS5A^hi (n≥31) CD8⁺ T cells: CD25 (left, P<0.0001, unpaired t-test) and CD44 expression (right, P<0.0001, unpaired t-test with the Welch correction) with representative histograms and (E) CD44⁺CD62L⁺ (left, P<0.0001, unpaired t-test with the Welch correction) and CD44⁺CD62L^- expression (right, P=0.0492, unpaired t-test).