Fig. 2.

NbSGT1 is required for cell death triggered by RipE1. (A) Effector-triggered cell death in silenced plants. Nicotiana benthamiana plants silenced for EV or NbSGT1 were infiltrated with Agrobacterium tumefaciens AGL1 strain carrying RipA1, RipE1, BAX, or GFP (as in Fig. 1). Photographs were taken 7 days post-infiltration. The number of patches showing cell death out of total infiltrated patches for that treatment in three independent experiments is indicated under each photograph panel. (B) Analysis of NbSGT1 gene expression in silenced plants. NbSGT1 and NbActin gene-specific fragments were amplified by reverse transcription polymerase chain reaction..