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Subcellular localization of the predicted nuclear localization signal-containing T3Es. Nicotiana benthamiana leaves were infiltrated with Agrobacterium tumefaciens AGL1 strain carrying YFP-tagged T3E and different organelle markers fused with mCherry (-mC) as indicated. Fluorescence was observed 2 days post-infiltration by confocal microscopy. Pictures are representative of three independent experiments. Scale bars = 25 μm (RipAD, RipAB, RipAO, and RipD), 50 μm (RipL), 10 μm (RipAF1).
