Table 1.
Ralstonia solanacearum type III effectors with predicted NLS
| Name | Gene IDa | Protein lengthb | Predicted NLS | Description | Reference | |
|---|---|---|---|---|---|---|
|
| ||||||
| NLStradamusc | cNLS Mapper (score)d | |||||
| RipAI | RSp0838 | 170 | 61 - KRRRKPETRQRLHRLRK - 77 | - | - | - |
| RipAB | RSp0876 | 174 | 112 - GKKKKKR - 118 | 93 - SGGKRKRDDE -112 (8); 112 - GKKKKKRDDE - 121 (5) | NLS harboring protein | Guéneron et al. (2000), Zheng et al. (2019) |
| RipAD | RSp1601 | 310 | 114 - ARAKSGKK - 121 | - | ||
| RipAF1 | RSp0822 | 350 | 33 - PPRRPKNR - 40 | - | Putative ADPribosyltransferase | |
| RipAY | RSp1022 | 416 | 26 - PRKKNGSPPRRA - 37 | - | γ-glutamyl cyclotransferase | Fujiwara et al. (2016), Mukaihara et al. (2016), Wei et al. (2017), Sang et al. (2018) |
| RipE1 | RSc3369 | 425 | 407 - RRRARRAALGK - 417 | - | - | - |
| RipAO | RSp0879 | 498 | 210 - RPAPMRQAARPAPPPARAP - 228 | - | - | - |
| RipD | RSp0304 | 643 | 184 - PRRKPS - 189 | - | - | - |
| RipH1 | RSc1386 | 765 | 466 - RPAGASGGMRRKHRKPGMR - 484 | - | - | - |
| RipA1 | RSc2139 | 1,063 | 91 - KPAPRRMRPPAAPGRKH - 107 | - | AWR motif | Solé et al. (2012) |
| RipA2 | RSp0099 | 1,127 | 88 - PKAP - 91 | - | AWR motif | Solé et al. (2012) |
| RipTAL | RSc1815 | 1,245 | 185 - RSARARR - 191; 980 - RIRR - 983 | 1107 - PHRKRPAETAI - 1117 (5.5) | Transcription activator-like protein | de Lange et al. (2013), Li et al. (2013), Schandry et al. (2016) |
| RipA4 | RSp0847 | 1,330 | 35 - KAPRK - 39; 832 - KRRLRHKLR - 840 | 683 - RKRQAVVEF - 691 (8); 829 - DQRKRRLRHK - 838 (6) | AWR motif | Solé et al. (2012) |
| RipL | RSp0193 | 1,403 | 1387 - QPSKSKGKGTKGKGKAK - 1403 | - | Pentatricopeptide Repeats | - |
| RipR | RSp1281 | 1,742 | 190 - KGKIRVKAR - 198; 944 - GRRKQRRPAKAK - 955 | - | - | - |
| RipS1 | RSc3401 | 2,353 | 27 - SKPQGHRAPRAR - 38 | 2311 - RERLLKKMRDA - 2321 (5) | - | - |
| RipS2 | RSp1374 | 2,483 | 93 - APRRQRSDREPPARQAKRARHDRDAIRTETGRARTAPAPA- 133; 135 - ARDRH - 139; 142 - SDARRRAP - 149 | 26 - HPRKRNRDP - 34 (5); 59 -PAPKRQRVS - 67 (7.5); 106 -RQAKRARHD - 114 (9); 2235 - PQVNKRRAQAK - 2245 (5) | SKWP repeats | - |
| RipS3 | RSp0930 | 2,291 | 1554 - RKKRRGQQRSFKPRK - 1568 | 108 - RIPLKRKREAG - 117 (10); 1550- VMDDRKKRRGQQRSF - 1564 (6) | SKWP repeats | - |
| RipS4 | RSc1839 | 2,570 | 2551 - PHRPQRPERPQQGKRAGHRP - 2570 | SKWP repeats | - | |
NLS, nuclear localization signal.
a
From GMI1000 in Ralsto T3E database (https://iant.toulouse.inra.fr/T3E).
b
Number of amino acid residue.
c
Predicted by NLStradamus (www.moseslab.csb.utoronto.ca/NLStradamus), prediction cutoff = 0.6.
d
Predicted by cNLS mapper (http://nls-mapper.iab.keio.ac.jp), cutoff score = 5.