TABLE. Primers used in quantitative real-time polymerase chain reaction (qPCR).
| Target gene (Accession number) | Sequence 5’-3’ (Forward and reverse) | Tm | CG % | Amplicon length (bp) |
| HDAC1 (NM_008228.2) | CCGGTTAGGTTGCTTCAATC | 59.6 | 50 | 118 |
| AACATTCCGGATGGTGTAGC | 59.8 | 50 | ||
| HDAC2 (NM_008229.2) | TATTATGGCCAGGGTCATCC | 59.6 | 50 | 119 |
| TCAGCAGTGGCTTTATGAGG | 59 | 50 | ||
| HDAC3 (NM_010411.2) | ATGCAGGGTTTCACCAAGAG | 60.1 | 50 | 117 |
| TGTTGCTCCTTGCAGAGATG | 60.1 | 50 | ||
| HDAC4 (NM_207225.2) | CCGCCAGCAGTTTTAAAGTC | 59.9 | 50 | 92 |
| ACCGAATGGAGATGCTCAAC | 60.1 | 50 | ||
| HDAC5 (NM_001077696.1) | ACTTTCCCCTCCGTAAAACG | 60.3 | 50 | 116 |
| AACAGTGCCATCCTTTCGAC | 60.1 | 50 | ||
| HDAC6 (NM_010413.3) | ATCTCAGCTGGCTTTGATGC | 60.5 | 50 | 116 |
| ATAATACGGCCACCAGCAAG | 60.0 | 50 | ||
| HDAC7 (NM_001204275.1) | ATGATGGCCTGGAACATAGG | 59.8 | 50 | 75 |
| GATGCTGCTGCAGAGAAATG | 59.7 | 50 | ||
| HDAC8 (NM_027382.4) | AGGGAATCTGAAGCATGTGG | 60.1 | 50 | 131 |
| CAAATTTCCCCTGCAGTCAC | 60.5 | 50 | ||
| HDAC9 (NM_001271386.1) | TTTGAGGTGGCAGAATCCTC | 60.2 | 50 | 106 |
| GAGCTGAAGCCTCATTTTCG | 60.1 | 50 | ||
| HDAC10 (NM_199198.2) | AACAGGAGCTGTGCACAATG | 59.9 | 50 | 143 |
| TCCTCTGCAGCCCATATTTC | 60.2 | 50 | ||
| HDAC11 (NM_144919.2) | TGATGGGGTTGAACACTGAG | 59.5 | 50 | 128 |
| AGCAGCCCCTTAAAAACTCC | 59.7 | 50 | ||
| Sirt1 (NM_019812.3) | GCCCTCAATTTCTGTTCTGC | 59.8 | 50 | 150 |
| TTTTGAGTGCTCCAGACACG | 60.0 | 50 | ||
| Sirt2 (NM_019812.3) | ACGGCTGCTCATTAACAAGG | 60.3 | 50 | 88 |
| GTCAAAATCCATGCCACCTC | 60.3 | 50 | ||
| Sirt3 (NM_001177804.1) | CATATGGGCTGATGTGATGG | 59.8 | 50 | 141 |
| AGATCTGCCAAAGCGAAGTC | 59.6 | 50 | ||
| Sirt4 (NM_001167691.1) | CGAGCAAAAGCTCCCAATAG | 60.0 | 50 | 145 |
| TTCCAGCCTTTGGACATCAG | 61.2 | 50 | ||
| Sirt5 (NM_178848.3) | CCAGCTTTAGCAGGAAAAGG | 59.1 | 50 | 139 |
| CCAGGTTTTCTCCAAACCAC | 59.4 | 50 | ||
| Sirt6 (NM_181586.3) | TGTCCAACACAGCTCCTTTC | 58.9 | 50 | 97 |
| CTTCCACATGTGTGGGATTC | 58.8 | 50 | ||
| Sirt7 (NM_001363439.1) | AGCTTCGGGATACCATTGTG | 60 | 50 | 104 |
| CAGGATTGTGTCTGCTTTGC | 59.4 | 50 | ||
| β-Actin (NM_007393.5) | TTGCTGACAGGATGCAGAAG | 61.1 | 50 | 147 |
| TGATCCACATCTGCTGGAAG | 59.8 | 50 |
bp: base pair; CG: cytosine and guanine content; HDAC: histone deacetylase 1-11; Sirt: Sirtuin 1-7; Tm (melting temperature) was calculated at default settings of 0.25 µM oligo concentration and 50 µM Na. Primers were designed using Primer3Plus software (http://www.bioinformatics.nl/cgi-bin/primer3plus/primer3plus.cgi).