Fig. 3: actin localisation in control and CMC-20 treated parasites. Actin localised to the cortex, flagella and around nuclei of control parasites (A, G). Control trophozoites did not stain for CWP-1 (D). In CMC-20 treated parasites actin was detected in the caudal flagella, the cortex and as condensed patches in the cytoplasm (B). It was also detected on the surface of cysts (C). CWP-1 was detected in ESVs (E) and on the surface of cysts (F). Co-localisation of CWP-1 with actin was observed (H, I). The nuclei are stained with DAPI (blue). F: flagella; C: cortex; N: nuclei; Bar: 5 μm.
