Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) | Thy1- alpha-synuclein (Line 61), BDF1 background | Rockenstein et al., 2002 | ASO (alpha-synuclein over-expressing) | With permission, UCSD |
| Strain, strain background (Escherichia coli) | Str. K12, MC4100 | Zhou et al., 2013 | WT (wild-type) | |
| Strain, strain background (Escherichia coli) | Str. K12, MC4100:ΔcsgBAC | Zhou et al., 2013 | ΔcsgBAC | |
| Strain (Escherichia coli) | Str. K12, MC4100: CsgA:Q49A/N54A/Q139A/N144A | Wang and Chapman, 2008 | SlowGo | |
| Antibody | Anti-alpha synuclein, mouse monoclonal | BD | Cat#: 610787 | (1:1000) |
| Antibody | Anti-pS129 alpha-synuclein, rabbit monoclonal | AbCam | Cat#: Ab51253 | (1:1000) |
| Antibody | Anti-tyrosine hydroxylase, mouse monoclonal | Millipore | Cat#: MAB318 | (1:1000) |
| Antibody | Anti-Iba1, rabbit | Wako | Cat#: 019–19741 | (1:1000) |
| Antibody | Anti-aggregated alpha-synuclein, rabbit polyclonal | AbCam | Cat#: MJFR-14-6-4-2 | (1:1000) |
| Antibody | Anti-mouse IgG-546, goat polyclonal | Life Technologies | Cat#: A-11003 | (1:1000) |
| Antibody | Anti-rabbit IgG-546, goat polyclonal | Life Technologies | Cat#: A-11010 | (1:1000) |
| Antibody | Anti-mouse IgG-488, goat polyclonal | Life Technologies | Cat#: A-11001 | (1:1000) |
| Antibody | Anti-rabbit IgG-HRP, goat polyclonal | Cell Signaling | Cat#: 7074 | (1:1000) |
| Peptide, recombinant protein | CsgA (N’-QYGGNN-C’) | Bio-Synthesis, Inc; Tükel et al., 2009 | CsgA | |
| Peptide, recombinant protein | N122A (N’QYGGNA-C’) | Bio-Synthesis, Inc; Tükel et al., 2009 | N122A | |
| Sequence-based reagent | 16S rRNA: 5’-TCCTACGGGAGGCAGCAGT-3’ and 5’-GGACTACCAGGGTATCTAATCCTGTT-3’ |
IDT | qPCR primer | PrimerBank |
| Sequence-based reagent |
rrsA:
5’-AGTGATAAACTGGAGGAGGTG-3‘ and 5’-GGACTACGACGCACTTTATGAG-3’ |
IDT | qPCR primer | PrimerBank |
| Sequence-based reagent |
csgA:
5’-ATGACGGTTAAACAGTTCGG-3’ and 5’-AGGAGTTAGATGCAGTCTGG-3’ |
IDT | qPCR primer | PrimerBank |
| Sequence-based reagent |
gapdh: 5’-CATGGCCTTCCGTGTTCCTA-3’ and 5’-CCTGCTTCACCACCTTCTTGAT-3’ |
IDT | qPCR primer | PrimerBank |
| Sequence-based reagent |
il6:
5’-TAGTCCTTCCTACCCCAATTTCC-3’ and 5’-TTGGTCCTTAGCCACTCCTTC-3’, |
IDT | qPCR primer | PrimerBank |
| Sequence-based reagent |
TH:
5’-CCAAGGTTCATTGGACGGC-3’ and 5’-CTCTCCTCGAATACCACAGCC-3’ |
IDT | qPCR primer | PrimerBank |
| Sequence-based reagent |
tnfa; 5’-CCCTCACACTCAGATCATCTTCT-3’ and 5’-GCTACGACAGTGGGCTACAG-3’ |
IDT | qPCR primer | PrimerBank |