Key resources table.
| Reagent type (species) or resource information |
Designation | Source or reference |
Identifiers | Additional | |
|---|---|---|---|---|---|
| Chemical compound, drug | Arginine-Vasopressin (AVP) | Tocris Bioscience, Bristol, UK | Cat# 2935/1 | ||
| Chemical compound, drug | Carbamoylcholine chloride | Tocris Bioscience, Bristol, UK | Cat# 2810/100 | ||
| Chemical compound, drug | CGS21680 | Tocris Bioscience, Bristol, UK | Cat# 1063/10 | ||
| Chemical compound, drug | Coelenterazine H substrate | Interchim, Montluçon, France |
Cat# R30783 | ||
| Chemical compound, drug | Compound 19 | Kindly synthetized by Domain Therapeutics Dzierba et al., 2015 |
|||
| Chemical compound, drug | DAMGO | Tocris Bioscience, Bristol, UK | Cat# 1171/1 | ||
| Chemical compound, drug | Forskolin | Tocris Bioscience, Bristol, UK | Cat# 1099 | ||
| Chemical compound, drug | IBMX | Tocris Bioscience, Bristol, UK | Cat# 2845/50 | ||
| Chemical compound, drug | Ionomycin calcium salt | Tocris Bioscience, Bristol, UK | Cat# 1704/1 | ||
| Chemical compound, drug | Metafectene PRO | Biontex, München, Germany | Cat#T040-5.0 | ||
| Chemical compound, drug | Morphine HCl | Francopia, Antony, France | |||
| Chemical compound, drug | Naloxone | Sigma-Aldrich, Saint-Quentin Fallavier, France | Cat# N7758 | ||
| Chemical compound, drug | Pertussis Toxin | Tocris Bioscience, Bristol, UK | Cat# 3097/50U | ||
| Chemical compound, drug | Quinpirole hydrochloride | Tocris Bioscience, Bristol, UK | Cat# 1061/10 | ||
| Chemical compound, drug | SKF81297 | Tocris Bioscience, Bristol, UK | Cat# 1447/10 | ||
| Chemical compound, drug | SNC80 | Tocris Bioscience, Bristol, UK | Cat# 0764/10 | ||
| Chemical compound, drug | Stromal cell-derived factor 1 (SDF-1) | Tocris Bioscience, Bristol, UK | Cat# 3951 | ||
| Chemical compound, drug | U50488H | Tocris Bioscience, Bristol, UK | Cat# 0495/25 | ||
| Material | Mithras² LB 943 Monochromator Multimode Microplate Reader | Berthold Technologies GmbH and Co. KG, Bad Wildbad, Germany | |||
| Material | MACSQuant10 flow cytometer | Miltenyi, Bergisch Gladbach, Germany | |||
| Material | Trans-Blot Turbo Transfer System | Bio-Rad, Hercules, California, USA. | |||
| Material | Odyssey CLx | LI-COR, Lincoln, Nebraska, USA | |||
| Material | LSM 700 laser scanning confocal microscope | Zeiss, Oberkochen, Germany | |||
| Material | Infrared floor | Videotrack; View Point, Lyon, France | |||
| Material | Computerized CPP boxes | Imetronic, Pessac, France | |||
| Material | Hot plate | Ugo Basile, Gemonio, Italia |
|||
| Antibody | GAPDH (14C10) Rabbit mAb | Cell Signaling, Leiden, Netherlands | Cat# 2118 | (1:2000) | |
| Antibody | Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP Rabbit mAb | Cell Signaling, Leiden, Netherlands | Cat# 4370 | (1:2000) | |
| Antibody | p44/42 MAPK (Erk1/2) (3A7) Mouse mAb | Cell Signaling, Leiden, Netherlands | Cat# 9107 | (1:2000) | |
| Antibody | Goat anti-rabbit IRDye800CW | LI-COR, Lincoln, Nebraska, USA | Cat# 926–3221 | (1:15000) | |
| Antibody | Goat Anti-mouse IRDye680CW |
LI-COR, Lincoln, Nebraska, USA | Cat# 926–68070 | (1:15000) | |
| Antibody | Goat polyclonal anti-HA HRP-conjugated antibody |
Bethyl Laboratories, USA | Cat#A190-138P | (1:10000) | |
| Antibody | Anti-HA tag antibody - ChIP Grade | Abcam, Cambridge, UK | Cat# ab9110 | (1:300) | |
| Antibody | Cy3 AffiniPure Donkey Anti-Rabbit IgG (H+L) | Jackson ImmunoResearch Europe Ltd, Cambridgeshire, Uk | Cat# 711-165-152 | (1:300) | |
| Cell line (Human) | HEK 293FT cell line | ThermoFisher Scientific Inc, Waltham, Massachusetts, USA | Cat# R70007 | ||
| Commercial assay or kit | SuperSignal ELISA Femto Maximum Sensitivity Substrate | ThermoFisher Scientific Inc, Waltham, Massachusetts, USA | (1:10) | ||
| Commercial assay or kit | Trans-Blot Turbo RTA Midi Nitrocellulose Transfer Kit | Bio-Rad, Hercules, California, USA | Cat# 1704271 | ||
| Commercial assay or kit | DAPI Hardset mounting medium | Vectashield Vector laboratories, Burlingame, California, USA | Cat# H-1500 | ||
| Strain, strain background (Mus musculus) | Gpr88+/+ and Gpr88-/-, hybrid 50% C57BL/6J–50% 129Sv genetic background | Meirsman et al., 2016a | |||
| Strain, strain background (Mus musculus) | C57BL/6JRj | Janvier Labs, Le Genest-Saint-Isle, France |