Figure 2. The 3D reconstruction of Arabidopsis flower from multiview imaging.

(A) MIPs of a 0.5 mm floral bud expressing ASY1:eYFP (green) and H2B:mRuby2 (magenta) viewed from eight different angles. Scale bar 200 µm. (B,C) Imaris MIP of 3D reconstructed flower. Longitudinal (D) and transversal (E) sections of the 3D reconstructed flower. (F) Surface rendered 3D model of the flower with indicated PMCs. (G) MIP of PMCs from the 3D model. Automated detection of PMCs using Imaris spot detection in one anther lobe is shown (41 PMCs were counted).

Figure 2—video 1. Animation of 3D reconstructed flower expressing ASY1:eYFP (green) and H2B:mRuby2 (magenta).

Download video file ^{(5.5MB, mp4)}

The movie shows rotation of the MIPs, cross-sections (partial MIPs) and surface renderings. Belongs to Figure 2B–D.