Key resources table.
Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Antibody | Monoclonal ANTI-FLAG M2 antibody produced in mouse | Sigma | Cat# F1804, RRID:AB_262044 |
IF(1:200) |
Antibody | Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 | Invitrogen | Cat# A-11029, RRID:AB_2534088 | IF(1:200) |
Antibody | Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 |
Invitrogen | Cat#: A-11008; RRID: AB_143165 | IF(1:200) |
Antibody | Polyclonal rabbit αCcmA | (Blair et al., 2018) | IF (1:200); WB (1:10,000) | |
Antibody | Polyclonal rabbit αMreB (H. pylori) | (Nakano et al., 2012) | IF (1:500); WB (1:25,000) | |
Commercial assay, kit | Click-iT Cell Reaction Buffer Kit |
Invitrogen | Cat# C10269 | |
Chemical compound, drug | Alexa Fluor 555 Azide, Triethylammonium Salt |
Invitrogen | Cat# A20012 | |
Chemical compound, drug | D-Ala-alk ((R)−2-Amino-4-pentynoic acid) | Boaopharma | Cat# B60090 | |
Chemical compound, drug | MurNAc-alk | (Liang et al., 2017) | ||
Chemical compound, drug | MurNAc | Sigma | Cat# A3007 | |
Chemical compound, drug | Wheat Germ Agglutinin, Alexa Fluor 488 Conjugate | Invitrogen | Cat# W11261 | |
Chemical compound, drug | Wheat Germ Agglutinin, Alexa Fluor 555 Conjugate | Invitrogen | Cat# W32464 | |
Other | ProLong Diamond Antifade Mountant | Invitrogen | P36961 |