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. 2020 Jan 9;9:e52482. doi: 10.7554/eLife.52482

Table 2. Strains used in this study.

Strain Genotype/description Construction Reference
LSH100 Wild-type: mouse-adapted G27 derivative - Lowenthal et al., 2009
LSH141 (Δcsd2) LSH100 csd2::cat - Sycuro et al., 2010
TSH17 (Δcsd6) LSH100 csd6::cat - Sycuro et al., 2013
LSH108 LSH100 rdxA::aphA3sacB - Sycuro et al., 2010
HMJ_Ec_pLC292-KU E. coli TOP10 pLC292-KU Transformation of TOP10 with pLC292-KU This study
HJH1 LSH100 rdxA::amgKmurU Integration of pLC292-KU into LSH108 This study
IM4 LSH100 mcGee:mreB Integration of pIM04into LSH100 This study
JTH3 LSH100 ccmA:2X-FLAG:aphA3 - Blair et al., 2018
JTH5 LSH100 ccmA:2X-FLAG:aphA3 rdxA::amgKmurU Natural transformation of HJH1 with JTH3 genomic DNA This study
KGH10 NSH57 ccmA::catsacB - Sycuro et al., 2010
LSH117 LSH100 ccmA::catsacB Natural transformation of LSH100 with KGH10 genomic DNA This study
SSH1 LSH100 ccmAI55A Natural transformation with ccmA I55A PCR product This study
SSH2 LSH100 ccmAL110S Natural transformation with ccmA L110S PCR product This study
LSH142 (ΔccmA) LSH100 ccmA::cat - Sycuro et al., 2010
JTH6 LSH100 rdxA::amgKmurU ccmA::cat Natural transformation of HJH1 with LSH142 genomic DNA This study