Figure 2. Immobilization of purified human sperm antigen and optimization of rabbit anti-human IgG antibody by biochip.

A duplicated dilution of sperm protein (0.09–100 µg/ml) on a N-hydroxysuccinimide (NHS)-modified biochip was tested (A). Similarly, optimized Cy3-based immunological assay was performed in a duplicated dilution of IgG antibody (0.09–100 µg/ml) (B).